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Size markers and Ladders

Online Catalogue | Nucleic Acid Research | Nucleic Acid Electrophoresis gel tanks and accessories | Size markers and Ladders

Phage Lambda DNA (0.05mg) Ref: BI-301005

Price: £7.00 / €10.85 (Excluding VAT at 17.5%)
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Phage Lambda c1857 Sam7 DNA is isolated from an infected E.coli strain W3350. Restriction enzyme digested lambda DNA (48,502bp) generates molecular weight size markers routinely used in gel analysis of nucleic acids.

Phage Lambda DNA (0.25mg) Ref: BI-301025

Price: £20.00 / €31.00 (Excluding VAT at 17.5%)
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Phage Lambda DNA (1mg) Ref: BI-301100

Price: £91.00 / €141.05 (Excluding VAT at 17.5%)
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Phage Lambda DNA/Hind III Markers (0.05mg) Ref: BI-302005

Price: £10.00 / €15.50 (Excluding VAT at 17.5%)
Quantity:

Lambda DNA (c1857 Sam7)/Hind III Markers are prepared by digesting Lambda DNA with Hind III, followed by heat-inactivation of the enzyme. The DNA fragments are then ethanol-precipitated and resuspended in the storage buffer.

Phage Lambda DNA/Hind II Markers (0.25mg) Ref: BI-302025

Price: £42.00 / €65.10 (Excluding VAT at 17.5%)
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Phage Lambda DNA/BstE II Markers (0.05mg) Ref: BI-303005

Price: £10.00 / €15.50 (Excluding VAT at 17.5%)
Quantity:

Phage Lambda (c1857 Sam7) DNA/BstE II are prepared by digesting Lambda DNA with BstEII followed by inactivation of the enzyme. The DNA fragments are then ethanol-precipitated and resuspended in the storage buffer

Phage Lambda DNA/BstE II Markers (0.25mg) Ref: BI-303025

Price: £42.00 / €65.10 (Excluding VAT at 17.5%)
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100bp DNA Ladder Ref: BI-304005

Price: £26.00 / €40.30 (Excluding VAT at 17.5%)
Quantity:

100 bp DNA Ladder is ideal for determining the size of double-stranded DNA from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.

Fragment sizes (base pairs):
1000, 900, 800, 700, 600, 500 x 2, 400, 300, 200, 100.

100 bp Blue eXtended DNA Ladder Ref: BI-304105

Price: £26.00 / €40.30 (Excluding VAT at 17.5%)
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100 bp BLUE eXtended DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing a dye which serves as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 - 1500 base pairs. The 500 bp fragment and the 1000 bp fragment are present at increased intensity to allow easy identification. All fragments are blunt-ended.

DNA fragments lenght (bp) and DNA mass:
100 (20 ng); 200 (20 ng); 300 (30 ng); 400 (50 ng); 500x2 (150 ng); 600 (80 ng); 700 (70 ng); 800 (110 ng); 900 (120 ng); 1000x2 (200 ng); 1500 (150 ng).

1kb DNA Ladder (0.05mg) Ref: BI-305005

Price: £26.00 / €40.30 (Excluding VAT at 17.5%)
Quantity:

The 1kb DNA Ladder is a convenient marker for determining the size of double stranded DNA from 250 to 10,000 base pairs. The ladder consists of 15 fragments that range in size from 250 to 10,000 base pairs. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide stained agarose gels and serve as reference indicatios. All fragments are blunt ended.

Fragment sizes (Kb)
10.0, 8.0, 6.0, 5.0, 4.0, 3.0x2, 2.5, 2.0, 1.5, 1.0x2, 0.75, 0.5, 0.25

1kb DNA Ladder (0.25mg) Ref: BI-305025

Price: £111.00 / €172.05 (Excluding VAT at 17.5%)
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100bp+1.5kb DNA Ladder (0.05mg) Ref: BI-306005

Price: £26.00 / €40.30 (Excluding VAT at 17.5%)
Quantity:

100bp DNA Ladder is ideal for determining the size of double stranded DNA from 100 to 1,500 base pairs. The ladder consists of ten fragments that range in size from 100-1,000bp in 100bp increments lus 1500bp fragment. The 500bp fragments is present at increased intensity to allow easy identification. All fragments are blunt ended.

The recommended amount of size marker to load on the gel is 0.4-0.6mg (2-3 µl) per lane. 1.7% agarose

Fragments at 1500, 1000, 900, 800, 700, 600, 500x2, 400, 300, 200, 100 bp

100bp+1.5kb DNA Ladder (0.25mg) Ref: BI-306025

Price: £111.00 / €172.05 (Excluding VAT at 17.5%)
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Phage Lambda DNA/Sty I Markers (0.05mg) Ref: BI-307005

Price: £10.00 / €15.50 (Excluding VAT at 17.5%)
Quantity:

Lambda DNA (c1857 Sam7)/Hind III Markers are prepared by digesting Lambda DNA with Sty I followed by inactivation of the enzyme. The DNA fragments are then ethanol-precipitated and resuspended in the storage buffer.

Phage Lambda DNA/Sty I Markers (0.25mg) Ref: BI-307025

Price: £42.00 / €65.10 (Excluding VAT at 17.5%)
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Phage T7 DNA (0.05mg) Ref: BI-310005

Price: £35.00 / €54.25 (Excluding VAT at 17.5%)
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Bacteriophage T7 DNA is isolated from an infected E.coli strain. DNA is linear, 39937 bp long. Some restriction enzyme has no recognition sequences on lambda DNA, therefore these enzymes are to be analysed on the other DNA substrate. T7 DNA is the most popular 'alternative' to lambda DNA substrate for the restriction endonucleases. The complete nucleotide sequence has been determined.

Phage T7 DNA (0.25mg) Ref: BI-310025

Price: £139.00 / €215.45 (Excluding VAT at 17.5%)
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Phage Lambda DNA dam, dcm (0.05mg) Ref: BI-313005

Price: £6.00 / €9.30 (Excluding VAT at 17.5%)
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Phage Lambda c1857 Sam7 DNA is isolated from an infected E.coli strain deficient in adenine and cytosine methylases (dam, dcm). Restriction endonucleases that are sensitive to dam and/or dcm methylation give complete digests with this DNA

Phage Lambda DNA dam, dcm (0.25mg) Ref: BI-313025

Price: £20.00 / €31.00 (Excluding VAT at 17.5%)
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pBR322/HaeIII Marker DNA (0.05mg) Ref: BI-312005

Price: £28.00 / €43.40 (Excluding VAT at 17.5%)
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pBR322 digest of pBR322 plasmid yields 22 fragments

pBR322/HaeIII Marker DNA (0.25mg) Ref: BI-312025

Price: £120.00 / €186.00 (Excluding VAT at 17.5%)
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pUC19/Mspl Marker DNA (0.05mg) Ref: BI-311005

Price: £30.00 / €46.50 (Excluding VAT at 17.5%)
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pUC19 digest of pUC19 plasmid yields 12 fragments

pUC19/Mspl Marker DNA (0.25mg) Ref: BI-311025

Price: £130.00 / €201.50 (Excluding VAT at 17.5%)
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pUC19 Plasmid DNA (0.05mg) Ref: BI-309005

Price: £33.00 / €51.15 (Excluding VAT at 17.5%)
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pUC19 is a commonly used E.coli plasmid cloning vector. The molecule is double stranded circle 2686 base pairs in length, pUC19 carries a multiple cloning site polylinker that contains unique sites for 13 different restriction endonucleases (6 cutters)

pUC19 Plasmid DNA (0.25mg) Ref: BI-309025

Price: £130.00 / €201.50 (Excluding VAT at 17.5%)
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Online Catalogue | Nucleic Acid Research | Nucleic Acid Electrophoresis gel tanks and accessories | Size markers and Ladders