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Protein Assays and Sample Preparations

Online Catalogue | Protein Research |  Protein Assays and Sample Preparations

Focus Signal, 50 preps Ref: BE786-250  


Price: £113.00 / €175.15 (Excluding VAT at 17.5%)
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A wide range of signalling proteins are located in specific membrane regions that are enriched with caveolin, cholesterol, glycolipids and glycosylphashpatidylinositol (GPI) regions known as rafts. The FOCUS™. Signal kit uses buffers that fractionate these specific regions or rafts away from other cellular organelles and components. The supplied strong chaotropic extraction buffer then extracts the proteins from the rafts and the resulting solubilised protein can be directly used in electrophoresis or other protein analysis techniques.

Focus GlycoProtein, 10 preps Ref: BE786-253  


Price: £166.00 / €257.30 (Excluding VAT at 17.5%)
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Based on lectin binding to specific glycoproteins with terminal a-D-glycosyl and a-D-glycosyl proteins. A spin column format containing a lectin bound resin with the capacity to bind ~5mg of glycoprotein is provided. The bound glycoproteins are rapidly eluted and ready for further analysis.

FOCUS PhosphoRICH, 5 preps Ref: BE786-255  


Price: £260.00 / €403.00 (Excluding VAT at 17.5%)
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A unique kit designed for the fractionation and enrichment of phosphoproteins and phosphopeptides from complex biological samples. Based on a spin column format for maximum efficiency, each column will bind ~20mg phosphorylated ovalbumin. Columns supplied with the kit can be re-used if regenerated and stored properly. The resulting fractions are suitable for electrophoresis, proteomic and cell signalling studies.

Protein DotMetric Basic Kit Ref: BE786-20  


Price: £134.00 / €207.70 (Excluding VAT at 17.5%)
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For Rapid Estimation of Protein Concentration
Uses a unique combination of proprietary reagents and test strips. No expensive equipment needed. The method involves mixing of 1µl of protein sample with a specific Dilution Buffer supplied with each kit. Apply 1µl of the solution to the test strip by point-of-contact capillary action. Under a specific buffer condition as protein enters into the matrix of the test strip it binds and saturates as protein diffuses in circular manner. Develop the test strip in 2-8 minutes. A circular protein spot is produced. The diameter of the spot is proportional to the concentration. By measuring the diameter of the spots with the dotMETRICTM scale, supplied with each kit, you can easily determine concentration of protein.

Focus Global Fractionation Kit Ref: BE786-018  


Price: £130.00 / €201.50 (Excluding VAT at 17.5%)
Quantity:  

1 Kit (number of preps dependent on sample size)

A complete fractionation kit designed to extract and solubilise mammalian proteins into five fractions - soluble proteins, hydrophobic membrane proteins, low hydrophobic proteins, cytoskeletal proteins and signaling membrane proteins that are concentrated in caveolin rich membranes and lipid rafts.
Focus Mitochondria KitFocus Mitochondria Kit Ref: BE786-022
1 Kit (number of preps dependent on sample size)

For researchers specifically interested in mitochondria studies and mitochondrial proteins, the FOCUS™ Mitochondria kit separates intact and active mitochondria from other cellular organelles. Approximately >90% of purified mitochondria have intact inner and outer membranes and are active.
Price: £155.00 / €240.25 (Excluding VAT at 17.5%)

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CB-X Protein Assay Kit Ref: BE786-12X  


Price: £126.00 / €195.30 (Excluding VAT at 17.5%)
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A Single Tube Protein Assay
More reliable protein estimation in less time than ever before. Saves time and reagents. CB-XTM Protein Assay provides both the single tube protein assay for rapid estimation and the conventional multi-tube calibration-plot assisted assays.Unlike Coomassie dye based protein assays, the CB-XTM Protein Assay is compatible with detergents and other common laboratory agents such as reducing sugars, thiols, and chelating agents. Suitable for a wide range of protein samples including detergent solubilized membrane proteins. No protein standard calibration is needed.CB-XTM is the only protein assay that can be used as "one sample and one assay reaction". No protein standard calibration needed. Quick and reliable estimation of protein concentration without preparing protein standard calibration plots. The CB-XTM Protein Assay is supplied with a batch specific " CB-XTM Table.

dotMETRIC A 1µl Protein Assay Ref: BE786-21  


Price: £221.00 / €342.55 (Excluding VAT at 17.5%)
Quantity:  

For Rapid Estimation of Protein Concentration
This kit is the same as BE-786-20 but also includes:

EASY-TO-USE SPOT APPLICATION DEVICE
For increased reproducibility and test reliability the dotMETRICTM kits are supplied with an optional Spot Application Device. The Spot Application Device allows application of samples using fixed volume capillary tips and simplifies the task of applying the protein solution on the test strips by point-of-contact capillary action. The Spot Application Device simplifies the application of one or more samples as well as improves reliability of results.

Focus Soluble & In Soluble, 50 preps Ref: BE786-247  


Price: £201.00 / €311.55 (Excluding VAT at 17.5%)
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A highly versatile fractionation kit that is compatible with a wide range of samples, including
mammalian cells and tissues, plants, yeast, bacteria and insect samples. The kit is designed to fractionate samples into soluble or hydrophilic, and insoluble or hydrophobic fractions. The resulting samples can be immediately used in electrophoresis or other analysis techniques.

Focus Cytoplasmic & Nuclear, 50 preps Ref: BE786-248  


Price: £201.00 / €311.55 (Excluding VAT at 17.5%)
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Specifically designed for the simple fractionation of cells and tissues into cytoplasmic and nuclear fractions. Based on the gentle lysis and centrifugation of cells to release nuclei and the subsequent extraction and solubilisation of proteins with a strong chaotropic agent. The cytoplasmic and nuclear fractions can be used for electrophoresis or other analysis techniques without further handling.
Focus SubCell KitFocus SubCell Kit Ref: BE786-260
1 Kit (number of preps dependent on sample size)

Includes all the reagents necessary for the fast and reliable fractionation of mammalian cell and tissue samples into enriched nuclear, mitochondrial, membrane and cytosolic fractions.

Majority of isolated mitochondria
contained intact inner and
outer membranes

Isolated mitochondria are roughly
90% active
Price: £196.00 / €303.80 (Excluding VAT at 17.5%)

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Non-Interfering Protein Assay Kit / 500 Assays Ref: BE786-005  


Price: £146.00 / €226.30 (Excluding VAT at 17.5%)
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Extensively tested to work in the presence of common lab agents, such as reducing agents (2ME, DTT), chelating agents EDTA, detergents (non-ionic, anionic, cationic and zwitterionic) amines (tris), sugar, urea, ammonium sulfate, guanidine hydrochloride, guanidine thiocyanate, drugs, antibiotics, cobalt and numerous other agents.
The Non-interfering Protein Assay is not affected even if you protein is in extration buffers containing [4M urea, 1% SDS, 100mM EDTA and 0.8% 2ME], [1% Sarcosy1, 4M guanidine thiocyanate, 10mM EDTA and 0.5% 2ME] and other strong extraction buffers.
Pre-Diluted BSA Standard (6x5ml)Pre-Diluted BSA Standard (6x5ml) Ref: BE786-114
BSA protein standards in an easy-to-use pre-diluted format. The Pre-Diluted Protein Standards allow faster and more reliable protein estimation. The Pre-Diluted Protein Standards are supplied in 6x 5ml aliquots ranging in concentration from 0.1 mg/ml 1.0 mg/ml.
Price: £23.00 / €35.65 (Excluding VAT at 17.5%)

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Bovine Albumin Standard 2x5ml Ref: BE786-006  


Price: £24.00 / €37.20 (Excluding VAT at 17.5%)
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BSA standard (2 mg/ml) prepared in saline buffer. Supplied as 2 x 5 ml size.

Bovine Gamma Globulin Standard (2mg/ml) Ref: BE786-007  


Price: £24.00 / €37.20 (Excluding VAT at 17.5%)
Quantity:  

g -Globulin standard (2 mg/ml) prepared in saline buffer. Supplied as 2 x 5 ml size.

Assay Tubes 2.0ml pk500 Ref: BE786-008  


Price: £45.00 / €69.75 (Excluding VAT at 17.5%)
Quantity:  

Protein assay tubes, 2ml reaction volumes. Allows proper mixing of assay reaction and good colour development.

Assay Cuvettes 1ml (Pk 500) Ref: BE786-009  


Price: £50.00 / €77.50 (Excluding VAT at 17.5%)
Quantity:  

Spectrophotometer assay cuvettes, 1ml reaction capacity, supplied as 500 cuvettes per box.

Online Catalogue | Protein Research |  Protein Assays and Sample Preparations

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