Sampe Pre Treatment

Proteases are present in almost all biological samples. Proteases are involved in processing cellular signals and post-translation modification of protein molecules. In cell free extracts proteases become a major concern for protein protection. When performing protein preparation it is often important to consider the role of proteases in your samples. In protein purification projects arresting proteolysis activity is critical otherwise the proteins of interest may be seriously damaged. Protease inhibitors are hence routinely used to protect proteins during purifcation schemes.

For Rapid Precipitation and Concentration. This kit is a simple to follow method for concentrating protein solutions by using a proprietary Universal Protein Precipitation Agent (UPPA) (Patents Pending). Protein solutions as dilute as 1ng/ml can be quantitatively concentrated into a small volume. Protein precipitation is not affected by the presence of detergents, chiaotropics, or other common laboratory agents. The method involves mixing protein solution with UPPA which quantitatively precipitates protein. The precipitate is washed and suspended in a small volume of a precipitate suspension buffer supplied with each kit and then the protein is ready for use. This kit is not suitable for those proteins which may lose their biological activity when precipitated. Recovery is generally 100%.

For running publication quality gels, the protein samples loaded on the gels should ideally have identical buffering conditions. If conditions vary, protein bands may distort during electrophoresis. Proteins containing excessive concentrations of salts, acids, bases and other agents are not suitable for running high quality gels. For dilute protein solutions, the concentration of the protein solution may be necessary before loading on the gels.

The PAGE-PerfectTM kit is a simple two step method for concentrating, cleaning and preparing protein solution for loading on gels. Simply treat (1-100ml) protein solution with Universal Protein Precipitation Agent (UPPA) which results in the quantitative precipitation of the protein solution. Protein precipitation is not affected by the presence of detergents, chiaotropics, or other common laboratory agents.

For iso-electric focusing (IEF) and 2D gels, the protein sample must be free from agents such as ionic detergents, metal ions, substrates, substrate analogs, inhibitors, and other charged molecules that may alter the net charge of protein molecules. For large screening projects, samples loaded on gel must be identically treated and be free from interfering agents; otherwise, the results may not be reproducible making interpretation difficult.

The Perfect-FOCUSTM kit provides a method for concentrating, cleaning and preparing protein solution for loading on IEF and 2D gels. Simply treat (1-100m) protein solution with UPPA (Universal Protein Precipitation Agent) which results in the quantitative precipitation of the entire protein in any solution. Protein precipitation is not affected by the presence of detergents, chiaotropics, or other common laboratory agents. The protein precipitate in IEF/2D sample loading buffer and load on gel.

A reagent kit for detection as well as estimation of SDS in a sample. A simple colour extraction method. Simply mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, blue colour is extracted in the extraction buffer.

2D protein analysis is like searching for a needle in the haystack. Proper strategy improves the chances of discovery. Fraction-Focus has been designed to improve the chances of discovery of less abundant and difficult to find proteins.

Fraction-FOCUSTM enables fractionation of protein samples and concentrates low abundant proteins into manageable fractions which consequently enhances the detectability of hard to find proteins. Fractionation minimizes the number of overlapping spots and enhances the number of protein spots detected in each step which simplifies protein identification. Allows creation of ASub-maps@ for a single sample.

The new NC EB kit improves the quality and yield from your sample.

The N& C-EB Kit has been developed for the extraction of cytoplasmic and nuclei fractions from cultured cells and tissues. The kit will allow a clean separation of cytoplasmic fraction from the nuclei for IEF/2D characterisation ,expression and transport studies. The nuclei fraction is solubilized in the reagents provided with the kit. The N &C-EB is based on Tris buffer, sodium & potassium salts and contains a mild detergent. Depending on application, additional agents such as protease inhibitors may be added into the NC-EBTM buffer.

Eliminates the major concern when dialysing samples, ie. the loss of samples due to transfer in and out of dialysis bags and handlings.

Allows dialysis of small samples without taking the sample out of the tube and eliminate loss.

Place your sample in the tube and close the cap adapted with dialysis membrane. Invert the Tube O Dialyzer in a dialysis tank and dialyze your samples. Salts and other molecules rapidly exchange across the dialysis membrane is complete, spin the tube for a brief 5 seconds and collect 100% of your sample.

Sample collection by spinning allows clean and 100% recovery. Eliminates the risks of leftover sample in the dialysis bag.

Pack of 50