DNA Ladder 100bp 1kb Ready-to-load Room Temperature Storage

Microlysis Plus DNA release reagent, 2x1ml, 100 preps
Ref: 2MLP-100

Price: £72.00 / €90.00
(Excl Vat)

Quantity:

Description

Protocol:
Mix cells with 20 µl microLYSIS®-PLUS
Overlay with mineral oil if necessary
Place in a Thermal Cycler

Cycling profile:

Step 1: 65°C for 15 mins*
Step 2: 96°C for 2 mins
Step 3: 65°C for 4 mins
Step 4: 96°C for 1 mins
Step 5: 65°C for 1 mins
Step 6: 96°C for 30 secs
Step 7: 20°C hold

After cycling, all or part of the microLYSIS®-PLUS / DNA mixture can be used directly in PCR. (It can make up to 40% of the final volume of most PCR mixtures). Or it can be stored at -20°C for future use.

*For very tough cells this incubation may need to be longer than 15 mins