DNA Ladder 100bp 1kb Ready-to-load Room Temperature Storage

MicroLYSIS DNA Release Reagent, 2x1ml, 100 preps
Ref: 2ML-100

Price: £72.00 / €90.00
(Excl Vat)

Quantity:

Description

Protocol:
Mix 1µl cells with 19 µl microLYSIS®
Overlay with mineral oil if necessary
Place in a Thermal Cycler

Cycling profile:

Step 1: 65°C for 5 mins*
Step 2: 96°C for 2 mins
Step 3: 65°C for 4 mins
Step 4: 96°C for 1 mins
Step 5: 65°C for 1 mins
Step 6: 96°C for 30 secs
Step 7: 20°C hold

After cycling, all of the microLYSIS® / DNA mixture can be used directly in PCR. Or it can be stored at -20°C for future use. For the amplification of bacterial/plasmid DNA, 1 to 3 µl of the microLYSIS® / DNA mixture is usually sufficient.