Perfect Focus for sample clean up prior to 2D gel (50 preps) Perfect Focus for sample clean up prior to 2D gel (50 preps) Ref: BE786-124
Price: £191.00 / €238.75
(Excl Vat)

Quantity:



Description
For iso-electric focusing (IEF) and 2D gels, the protein sample must be free from agents such as ionic detergents, metal ions, substrates, substrate analogs, inhibitors, and other charged molecules that may alter the net charge of protein molecules. For large screening projects, samples loaded on gel must be identically treated and be free from interfering agents; otherwise, the results may not be reproducible making interpretation difficult.

The Perfect-FOCUSTM kit provides a method for concentrating, cleaning and preparing protein solution for loading on IEF and 2D gels. Simply treat (1-100m) protein solution with UPPA (Universal Protein Precipitation Agent) which results in the quantitative precipitation of the entire protein in any solution. Protein precipitation is not affected by the presence of detergents, chiaotropics, or other common laboratory agents. The protein precipitate in IEF/2D sample loading buffer and load on gel.

Downloads


Perfect Focus 2D Prep Kit Instructions