Protein Expression, Extraction and Purification

Web Scientific has an extensive proteomics preparation range from protein expression media for bacterial cells, advanced homogenizers for all types of tissue and cell culture and a huge range of resins and beads for purification techniques.

What we offer:

Protein Expression

Web Scientific supplies materials and equipment for both bacterial expression and measurement of protein extracts in the form of Growth Media and Pre-cast Polyacrylamide gels.

Media used in protein expression studies are specially formulated to encourage the enhanced growth of transformed E.coli strains in order to maximise cell growth and protein yield. All of our culture medium is manufactured by Laboratorios CONDA, a European based company with over 50 years’ experience in creating high quality media products.
See our range of media here:

 

To check your protein expression, you’ll need to run a vertical PAGE gel, Web Scientific supplies a range of Precast Homogenous and Gradient Polyacrylamide Gels suitable for a variety of applications, including: Protein, DNA/RNA and peptide electrophoresis.

The gels we supply are presented in disposable cassettes with a format of 10 x 10cm fitting into most of the available mini-gel electrophoresis systems, including Cleaver, NOVEX, Serva, Hoefer, Biometra, Scie-Plas, etc. If in doubt please ask for compatibility. All of our polyacrylamide precast gels have a stacking gel and do not contain SDS. By choosing the appropriate running and sample loading buffer (with or without SDS) native, denaturing or reducing conditions can be created. Gels are available in boxes of x10. Combining different types of gels in one box is possible, please enquire.

All of our gels are manufactured by Anamed, Germany for which Web Scientific is the appointed UK Distributor. Gels are prepared to GLP standards using only the highest quality raw materials and then QC tested to guarantee the highest possible quality, meeting all essential requirements and specifications. Check out the full range here:

To run our precast or any handcast gels you'll need a vertical electrophoresis tank and power supply. Web Scientific distributes products from Cleaver Scientific Ltd, a UK based manufacturer of bench top electrophoresis systems. The Cleaver range features mini gel tanks compatible with our precast gels, as well as large format and specialist vertical electrophoresis tanks. Check it out here:

Protein Extraction

Web Scientific supplies a number of products designed to liberate proteins from a variety of different sources, from bacterial to mammalian tissues. These products include lysis and extraction buffers, in addition to the much needed protease inhibitors which protect proteins of interest during analysis. In addition, we also supply various homogenisers for the mechanical disruption of tissues.

Web Scientific is the exclusive UK distributor for Next Advance, an innovative equipment manufacturer focused on quality and affordability. Included in the Next Advance range are the Bullet Blender tissue homogenizer as well as a wide range of beads and lysis kits for all manner of samples, from microbial culture to bone!

The Bullet Blender® enables you to homogenize, disrupt, or lyse up to 24 tissue or cell culture samples at a time. Load the samples into standard polypropylene tubes, then place them in the Bullet Blender. The "bullets" in the instrument vigorously strike all of the tubes and homogenize your sample in minutes. The Bullet Blender homogenizer Gold models keep samples at 4°C!

Check out the video demonstration here:


WE also supply homogenizer beads in bulk packages as well as pre-filled bead lysis kits and empty tubes for every sample out there! Check out the full product range:

The Protein Extraction & Lysis Buffer (PE LB™) systems ensure good protein recovery, while maintaining their biological activity. The solubilized proteins are suitable for enzyme assays, electrophoresis, folding studies, chromatographic studies and many other downstream applications.

The PE LB™ systems offer a wide selection of buffers for lysis and extraction of proteins from bacteria, yeast, animal cells and tissues and insect cells. The PE LB™ systems are based on a proprietary combination of organic buffering agents, mild non-ionic detergents, and a combination of various salts to enhance extraction of proteins and maintain their stability of biological activities.

*PE LB™ is a trademark of G-Biosciences, 9800 Page Avenue, St. Louis, MO 63132-1429, USA.

Web Scientific supplies a range of protease inhibitor cocktails (ProteaseArrest family) as well as individual inhibitors used for the protection of protein integrity during extraction and purification.

The ProteaseArrest product range is comprised of highly efficient, liquid format general protease inhibitor cocktails that offer >95% protease inhibition. The range includes cocktails which are enhanced with inhibitors specific for individual species: Bacteria, Plants, Yeast, Fungi and Mammals.

*ProteaseArrest™ is a trademark of G-Biosciences, 9800 Page Avenue, St. Louis, MO 63132-1429, USA.

Protein Purification

Web Scientific offers a wide range of non-activated agarose beads for Size Exclusion & activation procedures, as well as a variety of activated beads for Affinity Chromatography and Pre-activated resins for coupling of affinity ligands (Immobilization). The catalog for separation media includes Low Pressure products for R&D scale, and High Pressure media (Rapid Run™ beads) for industrial scale separations.

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry which need to be separated. Affinity Chromatography is a technique that separates tagged proteins and other biomolecules using biological interactions. This technique is widely used to obtain high purity yield accompanied by good resolution and selectivity.

Web Scientific offers Affinity Chromatography resins in a variety of formats: Bulk resins, Gravity flow columns for low pressure applications and Cartridges for high pressure applications compatible with MPLC, FPLC and AKTA design systems.

Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins, etc.) to a support structure such as agarose beads that provides high stability and easier re-use of the immobilized molecule. The conjugation of affinity ligands and their use in chromatography have extended applications in many fields, including purification procedures, removal of contaminating substances, and biocatalysis. Web Scientific offers a range of pre-activated resins that are designed to couple ligands via stable & uncharged covalent linkages that minimize leakage of the affinity ligand and reduce nonspecific binding. Two different chemistries of pre-activated beads are provided:

  • Glyoxal beads for the covalent binding of agarose to lysine amino groups of the target ligand (similar to CNBr orientation)
  • Aminoethyl beads for the covalent binding of agarose to carboxyl amino acids group of the target ligand

Resins are available with different concentrations of agarose (4-6%) and different densities/concentrations of Glyoxal or Aminoethyl activated groups:

High and Very High activated resins for high recovery and yields:

  • High/Very high binding capacity
  • High immobilized enzyme stability
  • Possibility of multiple binding points
Low and Very Low activated resins for less non-specific binding:
  • Good binding capacity
  • Immobilized enzyme stability
  • Minimum distortion of immobilized enzyme

These resins are excellent options for research and industrial scale applications, conferring a qualitative advantage compared to CNBr agarose resins. The choice of Glyoxal or Aminoethyl will depend on the ligand to be immobilized, the accessibility of the reactive groups and the direction/orientation required for the binding to the support. The easiest strategy is to screen with the Glyoxal or Aminoethyl Test Kit to optimize the best choice of activation degree. These kits provide both High/Very High and Low/Very Low density beads.

Agarose is a very inert polysaccharide which forms hydrophilic and high gel strength gels at low concentrations. Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to the composition (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner.

Plain and crosslinked agarose beads are used in Gel Filtration Chromatography (or Molecular Size Exclusion) as well as for activating beads for biomolecule purification or immobilization. Plain and crosslinked agarose beads can be activated for ligand attachment due to their unique internal surface area and composition (inert polysaccharide). These beads are the basis for affinity chromatography resins such as Protein A & G, Glutathione, etc.

Please note: several of these resins are custom made, so please contact us for a price

Web Scientific offers a range of empty columns, cartridges and associated componenets to allow end users the opportunity to fill their own columns if/when required.