100bp 1.5kb DNA Ladder

Key Features

The 100 bp Marker bp is designed for use as molecular weight standard for agarose gel electrophoresis

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SKU: BI-3060XX Categories: ,
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Description

Stability tests / Quality Tests

Features: GeneON 100 bp DNA Plus Marker is composed of 11 individual DNA fragments which are re-dissolved in storage buffer.

Description/Preparation: The 100 bp PLUS DNA Ladder has a number of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for agarose gel electrophoresis.  The 100 bp DNA Ladder was designed for precise qualitative and approximately quantification of DNA mass. There are no unspecific bands besides the fragments. The 500bp fragment is used a reference band.

Usage: 1 µg/lane

Concentration:  0,2 µg/µl

Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl 

Tracking Dye: 6X DNA Loading dye required

Gel-picture: 1 μg of 100bp 1,5Kb DNA Ladder visualized by ethidium bromide staining on 1,7 % TAE agarose gel

Number of bands: 11 fragments

Number of bands and approx. DNA Mass:

 base pairs  100 200  300  400  500  600  700  800  900  1000  1500
 DNA mass ng  20  20  30  50  150  80  70  110  120  200 150


Loading: 
0.5 – max. 1.2 µg/ lane in 1.7 – 2.5 % Agarose. We recommend to add the loading dye in amount 1/10 from the DNA Ladder volume

Transportation: shipped on blue ice or room temperature

 

Storage: at -20°C for 24 months

 

 

 

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