5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2

Key Features

5x HOT FIREPol® Blend Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

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SKU: 5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2 Categories: ,
Special Requirements?



– Hot Start PCR


Mix Composition:

– HOT FIREPol® DNA polymerase

– Proofreading enzyme

– 5x Blend Master Mix Buffer

– 12. 5 mM MgCl2 1x PCR solution – 2.5 mM MgCl2

– 2 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

– BSA 

Advantages of Master Mix:

– decreased set-up time 

– reduced risk of contamination 

– less pipetting mistakes 

– consistent reaction to reaction performance 

– contains all reagents required for PCR 

Like all our products, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 12 months at 2-8ºC has no detrimental effects on the quality of 5x HOT FIREPol® Blend Master Mix.

SKU Pack Size Price
04-27-00125 1 ml (250rxn) £30.11
04-27-02025 20 ml (5000rxn) £561.84