5x HOT FIREPol® GC Master Mix

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SKU: 5x HOT FIREPol® GC Master Mix Categories: ,
Special Requirements?



– Hot Start GC-rich PCR 

– Fragment analysis

– TA cloning


Mix Composition:

– HOT FIREPol® DNA polymerase

– HOT FIREPol GC Buffer

– 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2

– 2 mM dNTPs

– BSA 


In separate vial:

100% DMSO

– 25 mM MgCl2


Advantages of Master Mix:

– decreased set-up time 

– reduced risk of contamination 

– less pipetting mistakes 

– consistent reaction to reaction performance 

– contains all reagents required for PCR 


Like all our products, the 5x HOT FIREPol® GC Master Mix is  for one month. This makes handling and set-up even more convenient, since no ice has to be used.


Product Performance:

The unique composition of the mix supports robust amplification of broad range of GC-rich targets. A total 14 GC-rich genes were amplified from human genomic DNA, using recommended reaction set-up and cycling protocol for  GC Master Mix.












Mix performs well with GC-rich  amplicons up to 3000 bp.

Good results are obtained also with Fragment Analyzis (DAT/SLC6A3).  


Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix.

SKU Pack Size Price
04-33-02015 20 ml £644.06
04-33-00115 1 ml £35.13