5x HOT FIREPol® GC Master Mix

Web Scientific is moving...

You can now find all Web Scientific products on our parent company's site, this site will be closing on 1st of June 2021. Please place all orders via Thistle Scientific.

Click the link below to see this product on our new website.

View Product
SKU: 5x HOT FIREPol® GC Master Mix Categories: ,
Special Requirements?

Description

Applications:

– Hot Start GC-rich PCR 

– Fragment analysis

– TA cloning

 

Mix Composition:

– HOT FIREPol® DNA polymerase

– HOT FIREPol GC Buffer

– 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2

– 2 mM dNTPs

– BSA 

 

In separate vial:

100% DMSO

– 25 mM MgCl2

 

Advantages of Master Mix:

– decreased set-up time 

– reduced risk of contamination 

– less pipetting mistakes 

– consistent reaction to reaction performance 

– contains all reagents required for PCR 

 

Like all our products, the 5x HOT FIREPol® GC Master Mix is  for one month. This makes handling and set-up even more convenient, since no ice has to be used.

 

Product Performance:

The unique composition of the mix supports robust amplification of broad range of GC-rich targets. A total 14 GC-rich genes were amplified from human genomic DNA, using recommended reaction set-up and cycling protocol for  GC Master Mix.
 

 

 

 

 

 

 

 

 

 

 

 

Mix performs well with GC-rich  amplicons up to 3000 bp.

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Good results are obtained also with Fragment Analyzis (DAT/SLC6A3).  
 

 

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix.

SKU Pack Size Price
04-33-02015 20 ml £644.06
04-33-00115 1 ml £35.13