Description
Applications:
– Hot Start PCR
– Multiplex PCR
Mix Composition:
– HOT FIREPol® DNA polymerase
– 5x MultiPlex Buffer
– 10 mM MgCl2 1x PCR solution – 2 mM MgCl2
– dNTPs
– BSA
– Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment
– Yellow dye Migration equivalent to 35-45 bp DNA fragment
– Compound that increases sample density for direct loading
Advantages of Master Mix:
– decreased set-up time
– reduced risk of contamination
– less pipetting mistakes
– consistent reaction to reaction performance
– contains all reagents required for multiplex PCR
Like all our products, the 5x HOT FIREPol® MultiPlex Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient since no ice has to be used.
Product Performance:
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® MultiPlex Mix.
