Bicinchoninic Acid (BCA) Protein Assay

£53.00£538.00 ex. VAT
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786-570-XX
SKU: 786-570-XX Category:
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Description

The Bicinchoninic Acid (BCA) Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions.

 
The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid (BCA) chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562 nm that is linear for increasing protein concentrations between the range of 0.02-2mg/ml. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations.
 
The Bicinchoninic Acid (BCA) Protein Assay is suitable for quantifying protein solutions in 1ml assays or in micro-wells.
 
The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.
 
For dilute protein samples (0.5-20µg/ml), we recommend the Micro Bicinchoninic Acid (BCA) Protein Assay
 
For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.

 

Features

  • Sensitive colorimetric assay
  • Linear range of 20-2000µg/ml
  • Compatible with wide range of ionic and non-ionic detergents
  • 1ml cuvette or micro well compatible

 

Applications

  • For the estimation of protein concentration

 

 

Additional information

Size

1000 Assays/ 5000 Micro-assays, 1gal, 1L, 250ml, 25ml, 500 Assays/ 2500 Micro-assays, 500ml

Description

BCA Solution (BCA Reagent A), Bicinchoninic Acid (BCA) Protein Assay, Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard, Copper Solution (BCA Reagent B)

SKU SizeDescription Price
BE786-570 500 Assays/ 2500 Micro-assays Bicinchoninic Acid (BCA) Protein Assay £139.00
BE786-571 1000 Assays/ 5000 Micro-assays Bicinchoninic Acid (BCA) Protein Assay £223.00
BE786-844 250ml BCA Solution (BCA Reagent A) £73.00
BE786-845 500ml BCA Solution (BCA Reagent A) £105.00
BE786-846 1L BCA Solution (BCA Reagent A) £159.00
BE786-847 1gal BCA Solution (BCA Reagent A) £538.00
BE786-848 25ml Copper Solution (BCA Reagent B) £53.00
BE786-890 500 Assays/ 2500 Micro-assays Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard £139.00
BE786-891 1000 Assays/ 5000 Micro-assays Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard £223.00