The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also termed as EAH-Agarose.
Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.
Molecules can be coupled to the free amine by numerous amine-reactive methods; however the use of the carbodiimide EDC allows coupling of free carboxyl groups. The resulting amide bond is highly stable and greatly reduces the chance of leaching of the affinity tag. The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification.
The Carboxyl Coupling Resin has a very high capacity of immobilized DADPA and offers >60µmole amine load for every ml of resin. This high capacity offers unsurpassed efficiency and capacity in the immobilization of carboxyl containing protiens.
For the complete kit (for generating 5 two milliliter affinity columns), view our Carboxyl Immobilization Kit.
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- High capacity EAH-Agarose/immobilized DADPA (Diaminodipropylamine).
- Capacity: Couple 1-2 mg peptide/ml resin
- Resin activated with >60µmol amine/ml resin
- 6% Cross-linked Agarose
- Long Spacer arm to limit steric hinderence (9Å)
- Immobilize carboxyl containing molecules
- Couple peptides for antibody purification
- Couple peptides & proteins to purify interacting molecules
- Couple nucleic acids or oligonucleotides through 5′-phosphate groups
- Generate affinity columns through carboxyl groups