Concanavalin A (Con A) Agarose

£84.00£767.00 ex. VAT
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786-208-XX
SKU: 786-208-XX Category:
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Description

Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method.  Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean).

 

Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues.  Con A agarose has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles.

 

As stated above, Con A is a metalloprotein and to maintain its binding characteristics the presence of both Mn2+ and Ca2+ is essential. Each subunit of Con A utilizes one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.
 

 

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Features

  • Binds α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues
  • Purification/ enrichment of of glycoproteins, polysaccharides and glycolipids
  • Ligand Density: 10-16mg Con A/ml resin
  • Capacity: 20-50mg thyroglobulin/ml resin
  • Bead structure: 4% agarose

 

Applications

  • Purification/ enrichment of of glycoproteins, polysaccharides and glycolipids

 

 

Additional information

Size

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Description

SKU SizeDescription Price
BE786-208 10 Columns Concanavalin A (Con A) Agarose £234.00
BE786-216 5ml resin Concanavalin A (Con A) Agarose £84.00
BE786-217 25ml resin Concanavalin A (Con A) Agarose £302.00
BE786-218 100ml Resin Concanavalin A (Con A) Agarose £767.00