Web Scientific is moving...

You can now find all Web Scientific products on our parent company's site, this site will be closing on 1st of June 2021. Please place all orders via Thistle Scientific.

Click the link below to see this product on our new website.

View Product
SKU: BC25-1-XX Category:
Special Requirements?


EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.


The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.


EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.


Click here to view our entire line of protein cross-linking and modification products.


Click here to see all of the cross-linking and modification accessories and tools available.


Click here to view our wide selection of protein cross-linkers.



  • EDC mediated coupling efficiency improved by N-hydroxysuccinimide
  • Allows for 2-step coupling process
  • Water soluble
  • CAS Number: 25952-53-8
  • Molecular Formula: C8H17N3•HCl
  • Molecular Weight: 191.71 g/mol
  • Spacer Arm (Å): 0
  • Reactive Group: Carbodiimide
  • Reactive Toward: Primary Amine + Carboxyl
  • Membrane Permeable: NO
  • Water Soluble: YES
  • Cleavable/Reversible: NO
  • Reaction pH: 4.7 to 6.0



  • Couple haptens or peptides to carrier proteins
  • Conjugate carboxyl to amine groups in peptides and proteins 
  • Cross link proteins to carboxyl surfaces and beads
  • Convert carboxyls to amine-reactive Sulfo-NHS esters
  • Activate nanoparticles to amine-reactive Sulfo-NHS esters
  • DNA Labeling through 5′ phosphate groups


Additional information


, , ,


SKU SizeDescription Price