EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.
The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.
EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.
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- EDC mediated coupling efficiency improved by N-hydroxysuccinimide
- Allows for 2-step coupling process
- Water soluble
- CAS Number: 25952-53-8
- Molecular Formula: C8H17N3•HCl
- Molecular Weight: 191.71 g/mol
- Spacer Arm (Å): 0
- Reactive Group: Carbodiimide
- Reactive Toward: Primary Amine + Carboxyl
- Membrane Permeable: NO
- Water Soluble: YES
- Cleavable/Reversible: NO
- Reaction pH: 4.7 to 6.0
- Couple haptens or peptides to carrier proteins
- Conjugate carboxyl to amine groups in peptides and proteins
- Cross link proteins to carboxyl surfaces and beads
- Convert carboxyls to amine-reactive Sulfo-NHS esters
- Activate nanoparticles to amine-reactive Sulfo-NHS esters
- DNA Labeling through 5′ phosphate groups