femtoCHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) washing buffer, femtoTBST™ Buffer.
In addition, optional AP labeled secondary antibodies against mouse and/or rabbit antibodies are supplied.
femtoCHROMO™ AP uses a modified BCIP (5-Bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
- Detects >5ng
- Single detection step
- High signal to background ratio
- High reproducibility