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SKU: 786-379-XX Category:
Special Requirements?


femtoCHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) washing buffer, femtoTBST™ Buffer.


In addition, optional AP labeled secondary antibodies against mouse and/or rabbit antibodies are supplied.


femtoCHROMO™ AP uses a modified BCIP (5-Bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.




  • Detects >5ng
  • Ready-to-use
  • Single detection step
  • High signal to background ratio
  • High reproducibility




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