Although the principle of ELISA is very simple, the optimization and perfection of the assay is not. To aid researchers, we manufacture a kit that contains all the crucial reagents necessary for a successful ELISA, including an enhanced blocking agent, washing buffer and an ultra sensitive colorimetric enzyme substrate.
femto-ELISA™ kits utilize a non-animal protein blocker, NAP-BLOCKER™ that minimizes cross-reactivity with researcher’s antigens and antibodies.
For the detection of HRP, we supply an improved, ultra sensitive, non-volatile, stable colorimetric substrate based on tetramethyl benzidine (TMB). Our reagent does not require hydrogen peroxide that can have detrimental effects on many biological factors, which may ultimately affect your assays. Figure 1 shows that our substrate is able to detect as little as 100pg of HRP.
- Choice of HRP or AP colorimetric substrates
- High sensitivity
- Non-animal blocking agent to minimize cross reactivity and lower background
- ELISA kits and substrates for highly sensitive colorimetric detection of HRP or AP.