femtoLUCENT™ PLUS-AP

Web Scientific is moving...

You can now find all Web Scientific products on our parent company's site, this site will be closing on 1st of June 2021. Please place all orders via Thistle Scientific.

Click the link below to see this product on our new website.

View Product
SKU: 786-10AP-XX Category:
Special Requirements?

Description

The femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity of antigens with no background. The kit reagents are sufficient for 25 mini blots (8.5 x 7.5 cm size).  

 

In addition, femtoLUCENT™ PLUS-AP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP BLOCKER™) and  wash buffer ().  The femtoLUCENT™ PLUS-AP kits allow detection of low femtogram levels (10-15) of antigens with low noise (signal/background) ratio.  The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane.  The trial size (Cat. # 786-10APT) is suitable for 5 mini blots or 300cm2.

 

For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat. 

 

Features

  • Intense light emission with low background and high signal
  • Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background
  • Economical: greater value compared to similar products

 

Applications

  • Quick and sensitive non-isotopic detection of proteins on transfer membranes (suitable for nitrocellulose & PVDF membranes)
  • Immunodetection of proteins and antigens for Western blots and dot blot applications
  • Low femtogram detection (10-15) that allows for detection of >10fg protein on a dot blot and >1pg on a Western blot

 

 

 

Additional information

Size

,

Description

,

SKU SizeDescription Price