FIREPol® DNA Polymerase

Key Features

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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SKU: FIREPol® DNA Polymerase Categories: ,
Special Requirements?

Description

Applications:

– Suited for a wide range of PCR assays

– Primer extension

– TA cloning

Concentration:

5 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

 

Reagents Provided:

– FIREPol® DNA Polymerase

– 10x Reaction buffer B (Mg2+ free) 

0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20

10x Reaction buffer BD (Mg2+ and detergent free)

0.8 M Tris-HCl, 0.2 M (NH4)2SO4

– 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.

SKU Pack Size Price
01-01-00500 500 units £16.49
01-01-02000 2000 units £61.24
01-01-01000 1000 units £31.52