FirstChoice™ Blocking Buffers contain a proprietary protein formulation that offers greater versatility and lack of cross-reactivity. This makes FirstChoice™ Blocking Buffers ideal as a first choice for optimization of new assays, system or when determining the optimal blocking buffer for elimination of non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. FirstChoice™ Blocking Buffers are compatible with antibodies and avidin/biotin based systems and results in high signal to background ratios.
For users convenience FirstChoice™ Blocking Buffers are supplied in widely used TBS (Tris-buffered saline at pH 7.5) and PBS (phosphate-buffered saline at pH 7.5) buffers as well as in separate formulations containing Tween®-20 for improving blocking efficiencies.
- For Western blotting and ELISA
- Available as TBS or PBS with optional Tween®-20
- Animal serum free
- Biotin free
- Ideal blocking buffer for setting up new assays and systems