FOCUS™ Mitochondria

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SKU: BE786-022 Category:
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Description

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

Specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria.  The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality. 
This kit is highly adaptable for use with animal tissues and other sources of mitochondria. The kit is designed for 50 preps, where one prep is equivalent to 2×107 mammalian cells or 100mg mammalian tissue.

 

Features

  • Fast and efficient fractionation of active heavy & light mitochondria
  • Suitable for cultured animal cells and tissues

 

Applications

  • Fraction of mammalian cells and tissues into a mitochondrial rich fraction
  • Suitable for sample preparation for 2D gel electrophoresis and other applications

Additional information

Size