FOCUS™ ProteaseArrest™

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SKU: BE786-108F Category:
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Description

FOCUS™ ProteaseArrest™ is a ready-to-use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.
 
The protease inhibitor cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases. Due to the optimized concentration of the various inhibitors, the FOCUS™ ProteaseArrest™ shows excellent inhibition of protease activities, and is therefore suitable for the protection of protein samples from animal tissues, plants, yeast and bacteria.
 
FOCUS™ Protease Arrest™ is compatible with 2D electrophoresis as it uses an alternative to EDTA as an inhibitor of metalloproteases. The absence of EDTA allows for optimal action of nucleases or removing nucleic acids from the samples. In addition, FOCUS™ ProteaseArrest™ uses PMSF as its primary serine protease inhibitor as opposed to the commonly used Pefabloc®. Pefabloc® has been reported to modify proteins at high concentrations and result in artifacts in subsequent 2D electrophoresis and mass spectrometry.
 

ProteaseArrest Family

Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition.

 

The ProteaseArrest™ family of inhibitor cocktails also includes species specific inhibitor cocktails.  These cocktails are enhanced with inhibitors specific for the species.  Species specific cocktails are available for Bacteria, Plant, Yeast, Fungus and Mammalian samples.

 

Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification.

 

Features

  •  2D electrophoresis compatible, broad spectrum protease inhibitor cocktail.
  •  100X concentrated, ready-to-use solution.
  •  High inhibition levels: 1X FOCUS™ ProteaseArrest™ inhibits >95% of protease activities (i.e. Mouse Pancreas Extract, 0.5mg/ml protein).

 

Applications

  •  Inhibition of protease activity in protein preparations.
  •  Suitable for 2D gel sample preparation for protection of protein samples.

 

 

Additional information

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