The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
Proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation.
Preparation of protein samples for 2D electrophoresis.
Blocking of thiols for preventing re-oxidation of proteins.