FOCUS™ SubCell Kit

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SKU: BE786-260 Category:
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Description

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.

 

FOCUS SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins. The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes. The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements.
 
FOCUS SubCell is suitable for cultured animal cells and can be adapted for animal tissues. The kit is designed for 50 preps, where one prep is equivalent to 2×107 mammalian cells or 100mg mammalian tissue.

 

Features

  • Adaptable for animal tissue
  • Resulting fractions suitable for 2D electrophoresis and other applications
  • Isolated mitochondria are ~90% active

 

Applications

  • Fractionation of cells and tissues into mitochondrial, nuclear, cytosolic and membrane proteins
  • Suitable for proteomic and cell signalling studies

Additional information

Size