G-Sep™ DEAE Agarose Fast Flow

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SKU: 786-967-XX Category:
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G-Sep™ DEAE Agarose Fast Flow (FF) resin is a weak anion exchanger composed of highly cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups.
The resin is extensively used for biomacromolecule separations in both research and industrial applications. The G-Sep™ DEAE Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols and have been the industrial standard for ion exchange chromatography during recent decades.
Click here to request bulk or custom sizes.
Other G-Sep™ Ion Exchange resins include the weak cation exhanger CM, the strong anion exhanger Q and the strong cation exhanger  SP.


  • Matrix: Cross-linked agarose beads, 6%
  • Ligand: Diethylaminoethyl
  • Ion Exchanger : Weak anion exchanger
  • Bead form: Spherical, diameter 50-160μm
  • Ionic Capacity: 0.11-0.16 mmol (H+)/ml
  • Binding Capacity: 90 mg HSA /ml medium
  • pH stability Working Range: 2-9
  • pH stability Cleaning-in-Place (CIP): 2-14
  • Maximum Flow Velocity: 450cm/h
  • Maximum Pressure: 0.3MPa
  • Exclusion limit(globular proteins): 4 x 106
  • Physical Stability: Negligible volume variation due to changes in pH or ionic strength
  • Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
  • Autoclavable: With Cl as counter Ions, at 121°C, pH 7, for 30 min in 0.2M sodium acetate for autoclaving
  • Storage Conditions: 4 to 30°C, 20% Ethanol



  • For ion exchange chromatography



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