Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. G-Sep™NI NTA Agarose Fast Flow is specifically designed for the purification of recombinant proteins fused to the 6x histidine (6XHis) tag.
The resin is specifically designed for the purification of recombinant proteins fused to the 6X histidine (6XHis) tag expressed in bacteria, insects, and mammalian cells. The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.
G-Sep™ NI NTA Agarose Fast Flow (FF) resin is nickel ions immobilized onto highly cross-linked 6% agarose beads. The G-Sep™ NTA Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. G-Sep™ Ni NTA Agarose Fast Flow (FF) resin using cobalt is also available
- Matrix: Cross-linked agarose beads, 6%
- Bead form: Spherical, diameter 50-160μm
- Spacer: Epichlorohydrin
- Chelating Agent: nitrilotriacetic acid (NTA)
- pH stability Working Range: 3-12
- pH stability Cleaning-in-Place (CIP): 2-14
- Maximum Flow Velocity: 450cm/h
- Exclusion limit(globular proteins): 4 x 106
- Physical Stability: Negligible volume variation due to changes in pH or ionic strength
- Chemical Stability: Stable to all commonly used aqueous buffers: 6M urea, 8M guanidine hydrochloride,
- Autoclavable: 121°C, pH 7, for 30 min
- Storage Conditions: 2 to 8°C, 20% Ethanol