G-Sep™ Phenyl Agarose Fast Flow

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SKU: 786-959-XX Category:
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G-Sep™ Phenyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with phenyl groups for Hydrophobic Interaction Chromatography (HIC).   The resin is used to separate biomolecules on the basis of relative hydrophobicity.  


G-Sep™ Phenyl Agarose Fast Flow is available in two levels of ligand substitution degree to help to find the optimal selectivity and binding capacity for a given application.


G-Sep™ Agaroses for HIC are also available with butyl and octyl groups immobilized on 6% cross-linked agarose beads through neutral and highly stable ether linkages.


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  • Matrix: Highly cross-linked Agarose, 6%
  • Bead form: Spherical, diameter 50-160μm
  • Ligand: Phenyl
  • Ligand Concentration: About 25μmol/ml or 40μmol/ml for low and high sub respectively
  • Binding Capacity: About 20mg HSA/ml resin or 20mg HSA/ml resin for low and high sub respectively
  • pH stability Working Range: 3-13
  • pH stability Cleaning-in-Place (CIP): 2-14
  • Maximum Pressure (MPa): 0.3
  • Maximum Flow Velocity: 450cm/h
  • Exclusion Limit (Globular Proteins): 4 x 106
  • Physical Stability: Negligible volume variation due to changes in pH or ionic strength
  • Chemical Stability: Stable to all commonly used aqueous buffers: 1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
  • Sterilization: Autoclavable, In 1M NaOH,121 °C, pH 7, for 30 min
  • Storage Conditions: 4 to 30°C, 20% Ethanol



  • Hydrophobic Interaction Chromatography



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