G-Sep™ SP Agarose Fast Flow

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SKU: 786-971-XX Category:
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Description

G-Sep™ SP Agarose Fast Flow (FF) resin is a strong cation exchanger composed of highly cross-linked 6% agarose beads, with Sulphopropyl (SP) strong cation exchange groups.
 
The resin is extensively used for biomacromolecule separations in both research and industrial applications. The G-Sep™ SP Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols and have been the industrial standard for ion exchange chromatography during recent decades.
 
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Other G-Sep™ Ion Exchange resins include the weak anion exhanger DEAE, the weak cation exchanger  CM and the strong anion exhanger Q.
 

Features

  • Matrix: Cross-linked agarose beads, 6%
  • Ligand: Sulphopropyl
  • Ion Exchanger : Strong cation exchanger
  • Bead form: Spherical, diameter 45-165μm
  • Ionic Capacity: 0.18-0.25 mmol (Na+)/ml
  • Binding Capacity: 70 mg lysozyme /ml medium
  • pH stability Working Range: 2-12
  • pH stability Cleaning-in-Place (CIP): 2-14
  • Maximum Flow Velocity: 450cm/h
  • Maximum Pressure: 0.3MPa
  • Exclusion limit(globular proteins): 4 x 106
  • Physical Stability: Negligible volume variation due to changes in pH or ionic strength
  • Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
  • Autoclavable: With Na+ as counter Ions, at 121°C, pH 7, for 30 min in 0.2M sodium acetate for autoclaving
  • Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate

 

Applications

  • Ion exchange chromatography

 

 

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