G-Trap™ Ion Exchange Selection Kit

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SKU: BE786-1018 Category:
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Description

G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and/or pH or ionic strength optimization of start and elution buffer to separate the target protein or substance via ion exchange chromatography.

Biomolecules or proteins that carry net charge opposite to that of ion-exchanger bind to that ion exchanger and are thus separated from bulk molecules in the sample.

 

G-Trap™ Ion Exchange Selection Kit contains one of each, G-Trap™ CM Agarose Fast Flow, G-Trap™ SP Agarose Fast Flow, G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column.

Once initial screening for suitable ion exchange resin is done by G-Trap™ Ion Exchange Selection Kit, indiviaul G-Trap™ Ion Exchange Agarose Fast Flow Column can be ordered in bulk as per requirement.

 

G-Trap™ Ion Exchange Agarose Fast Flow Columns  are available in 1 ml and 5 ml Column Volume namely G-Trap™ CM Agarose Fast Flow, G-Trap™ SP Agarose Fast Flow, G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column..

 

G-Trap™ Ion Exchange Agarose Fast Flow Columns are ready to use prepacked columns for separation of biomolecules on the basis of charge. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method scouting, separation or purification of biomolecules, sample concentration and sample cleanup for charged molecules. 

 

The  properties of the G-Trap™ Ion Exchange Selection Kit resin and the column are listed below

 

G-Trap™ Ion Exchange Selection Kit resin

 

G-Trap™ CM Agarose Fast Flow resin

Ion Exchanger: Weak cation exchanger

Matrix: Cross-linked agarose beads, 6%

Bead size: 45-165 µm

Ligand: Carboxymethyl

Ionic Capacity: 0.09-0.13 mmol (H+)/ml

Binding Capacity: 50 mg lysozyme/ ml medium

pH stability Working Range: 4-12

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 450cm/h

Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively

Maximum Pressure: 0.3MPa

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol

 

G-Trap™ SP Agarose Fast Flow resin

Ion Exchanger: Strong cation exchanger

Matrix: Cross-linked agarose beads, 6%

Bead size: 45-165 µm

Ligand: Sulphopropyl

Ionic Capacity: 0.18-0.25 mmol (Na+)/ml

Binding Capacity: 70 mg lysozyme /ml medium

pH stability Working Range: 2-12

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 450cm/h

Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively

Maximum Pressure: 0.3MPa

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate

 

G-Trap™ DEAE Agarose Fast Flow resin

Ion Exchanger: Weak anion exchanger

Matrix: Cross-linked agarose beads, 6%

Bead size: 45-165 µm

Ligand: Diethylaminoethyl

Ionic Capacity: 0.11-0.16mmol (Cl)/ml

Binding Capacity: 90mg HSA/ml medium

pH stability Working Range: 2-9

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 450cm/h

Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively

Maximum Pressure: 0.3MPa

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol

 

G-Trap™ Q Agarose Fast Flow resin

Ion Exchanger: Strong anion exchanger

Matrix: Cross-linked agarose beads, 6%

Bead size: 45-165 µm

Ligand: Quaternary ammonium

Ionic Capacity: 0.18-0.25mmol (Cl)/ml

Binding Capacity: 120 mg HSA /ml medium

pH stability Working Range: 2-12

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 450cm/h

Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively

Maximum Pressure: 0.3MPa

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol

 

G-Trap™ Ion Exchange Selection Kit, 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

Application(s): 

  • G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and/or pH or ionic strength optimization of start and elution buffer to separate the target protein or substance via ion exchange chromatography. Biomolecules or proteins that carry net charge opposite to that of ion-exchanger bind to that ion exchanger and are thus separated from bulk molecules in the sample. 

 

Additional information

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