G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub)

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SKU: 786-1007-XX Category:
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Description

G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC). 

 

Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of surface-exposed hydrophobic amino acids present on the protein or enzyme to be separated and the concentration and type of salt used in binding or start buffer. In HIC, the binding of biomolecules to the column (ligand) is facilitated by using higher concentrations of anti-chaotropic salts such as ammonium sulfate, sodium sulphate etc in the binding buffer or start buffer.

 

Straight alkyl chains ligands such as butyl, octyl show pure hydrophobic character whereas aryl ligands such as phenyl show mixed mode behavior where aromatic, hydrophobic as well as lack of charge play role in protein/biomolecules adsorption to the column.

 

G-Biosciences offer a wide range of G-Trap™ HIC Columns such as G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) so that one can choose the HIC column that is suitable for separation of the target protein. In addition G-Trap™ HIC Selection Kit is available for initial screening for appropriate hydrophobic column and purification conditions for the target protein

 

The properties of G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) resin and column are listed below

 

G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) Resin

Mean particle size: 90 µm

Bead structure: Highly cross-linked 6% Agarose

Ligand: Phenyl

Ligand Concentration: About 25 μmol/ml

Binding Capacity: About 20 mg HSA/ml resin

pH stability Working Range: 3-13

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Pressure (MPa): 0.3

Maximum Flow Velocity: 450 cm/h

Exclusion Limit (Globular Proteins): 4 x 106

Physical Stability: Negligible volume variation due to changes in pH or ionic strength

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol

 

G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub), 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub), 5 ml columns

Column Volume: 5 ml

Columns Dimension: 1.6 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

Application(s):

  • G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) are prepacked ready to use columns for separation of biomolecules via Hydrophobic Interaction chromatography (HIC). 

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