GET™ Total RNA

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SKU: BE786-132 Category:
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Description

The GET™ Total RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA Spin Columns.  The <60-minute protocol is simple; after homogenization, RNA is bound to the GET™ RNA Spin Columns and washed.  The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from the column. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays. 
 
Three step protocol:
  • Arrest™ Extraction Buffer lyses samples instantly destroying RNase activity.
  • GET™ RNA Spin Columns captures RNA.
  • After a brief washing step, pure and protein free RNA is eluted.

 

Features

  • Rapidly inhibit destructive RNases
  • Strong chaotropic buffer for rapid RNA release
  • High affinity RNA binding spin columns
  • Optional DNase treatment
  • Compatible with numerous species and tissues
  • Suitable for animal and plant tissues, cultured cells, blood and bacteria
  • Suitable for 50 x 50mg extractions

 

Applications

  • For the extraction and purification of protein and DNA free RNA.

 

 

Additional information

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