HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting.
MOLECULAR WEIGHT: 606.69
SPACER ARM (Å): 24.3
REACTIVE GROUP: sulfo-NHS-ester
MEMBRANE PERMEABLE: NO
WATER SOLUBLE: YES
CLEAVABLE/ REVERSIBLE: YES
REACTIVE pH: 7-9
Following labeling, cells are lysed with Mammalian Cell PE LB™, a buffered lysis solution that employs a mild non-ionic detergent for enhanced extraction and stability of proteins, and the cell lysate is applied to a Streptavidin agarose column. Unlabeled intracellular proteins are washed away and the biotin labeled cell surface proteins are then released by reduction of the disulfide bond with DTT.
The kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines. This kit is supplied with sufficient reagents for five experiments, with each experiment consisting of four 90-95% confluent T-75cm2 flasks.
- Complete cell surface biotin labeling and isolation.
- Suitable for a wide selection of mammalian cells.
For the isolation of cell surface proteins.
For the study of cell surface trafficking.
- For the study of receptor:ligand interactions.