HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by passing clarified lysate through prepacked columns.
Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal force.
HOOK™ GST Protein Purification kit is optimized to yield up to 10mg/250ml culture of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein.
For small scale, rapid GST protein isolation from bacteria we recommend our spin format kits.
- Isolate >10mg GST tagged proteins from ~250ml bacteria
- No sonication required, supplied with lysis buffer and enzyme
- For the isolation of GST tagged recombinant proteins from bacteria