HOOK™-Sulfo-NHS-SS-Biotin Labeling Kit

Web Scientific is moving...

You can now find all Web Scientific products on our parent company's site, this site will be closing on 1st of June 2021. Please place all orders via Thistle Scientific.

Click the link below to see this product on our new website.

View Product
SKU: BEBS-09 Category:
Special Requirements?

Description

HOOK™-Sulfo-NHS-SS-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are available on the surface of the molecules and can be reacted with amine reactive esters.

 

The kit is designed for the conjugation of biotin to protein primary amine groups and is supplied with 25mg HOOK™-Sulfo-NHS-SS-Biotin, a specific Optimizer Buffer™, for enhanced conjugation, Spin-OUT™ columns, for purification of labelled protein, and reagents to determine the amount of biotinylation. 

 

Each kit is designed for the coupling of 1-10mg protein in 1ml buffer, suitable for 10 couplings.

 

Also available in a micro size kit for the coupling of 1-10mg protein in 1ml buffer, suitable for 10 couplings.

 

This product is an improvement of BK-09 and its direct replacement.

 

Features

  • Complete kit for labeling protein primary amines
  • Optimizer Buffer for improved coupling efficiency
  • Gel filtration columns for rapid (<10 minute) purification
  • Biotin assay reagents to determine level of biotin incorporation
  • Labels 1-10mg protein/reaction
  • Suitable for 10 coupling reactions

 

 

 

Additional information

Size