Luciferase (Firefly; Recombinant)

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SKU: 786-1308-XX Category:
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Description

Firefly luciferase is a unique photoprotein used in bioluminescence for high sensitive detection and quantification of ATP and as a reporter for studying gene function and regulation.  Luciferase (Firefly, Recombinant) is recombinant 62 kDa protein expressed in E. Coli.  

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Highlights

  • Single band purity, >99 % by SDS-PAGE
  • High specific activity: ≥1 x 10E10 units/mg   

Features

  • Molecular weight: 62 kDa
  • Purity: >99 % by SDS-PAGE. Single band purity
  • Protein amount: determined based on absorbance at 280 nm and molecular extinction co-efficeint of Luciferase
  • Source: E. coli
  • Shipping and handling: Luciferase (Firefly, Recombinant) is shipped on dry ice. Store at -20°C. Avoid repeated thawing and thaw on ice. When stored properly as advised the enzyme is stable for 1 year. For long term storage make small aliquotes and store at -70°C. For making enzyme dilutions it is recomended to add 1 % carrier protein such as BSA to the dilution buffer.

Application(s)

  • Firefly luciferase is used in bioluminescence for high sensitive detection and quantification of ATP
  • Firefly luciferase is used as reporter for studying gene function and regulation.

Additional information

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