Lumino™ Firefly Luciferase Glow Assay

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SKU: 786-1353-XX Category:
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Description

Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates.

Lumino™ Firefly Luciferase Glow Assay is homogenous, high-sensitive, glow type (long-lived) bioluminescent assay with signal half-life of more than 3 hrs.
A long signal half-life (>3hrs) of the luminescent assay is necessary for batch-processing (HTS) systems, where a large number of plates can be stacked after assay and before measurement.

 

Features:

  • High sensitivity: High sensitive detection of firefly luciferase activity. 
  • Suitable for HTS assays: Designed for batch-processing of mammalian cells. Single reagent is added for cell lysis and luminescent assay which makes HTS assays faster to perform.
  • Stable signal: Glow type bioluminescent assay with signal half-life of greater than 3 hrs. 
  • Excellent linearity: Linear over high-log order (5 or more) of luciferase concentration. The linear range is also dependent of the sensitivity of the luminometer.
  • Assay reagents compatible with other firefly luciferases.

Application(s):

  • Rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme activity.
  • Suitable for high throughput screening or quantification of luciferase expression in mammalian cells as its glow type bilumnescent assay with signal half life of more than 3 hours.

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