The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult Macaque (primate) brains.
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Blot lane order:
- Protein marker
- Macaque Frontal Cortex Lysate
- Macaque Posterior Cortex Lysate
- Macaque Cerebellum Lysate
- Macaque Hippocampus Lysate
- Macaque Olfactory Bulb Lysate
- Macaque Striatum Lysate
- Macaque Thalamus Lysate
- Macaque Midbrain Lysate
- Macaque Entorhinal Cortex Lysate
- Macaque Pons Lysate
- Macaque Medulla Lysate
- Macaque Spinal Cord
- Macaque Whole Brain Lysate