The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
The premade blots are prepared with the appropriate GenLysate™ and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient™ Western Transfer Buffer [20X].
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate™.
Blot lane order:
- Protein marker
- Mouse Whole Liver
- Mouse Nuclei Lysate
- Mouse Mitochondria Lysate
- Mouse Golgi Complex Lysate
- Mouse Smooth Endoplasmic Reticulum Lysate
- Mouse Rough Endoplasmic Reticulum Lysate
- Ready-to-screen western tissue blot