OmniPrep™ for Yeast

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SKU: BE786-400 Category:
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Description

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

 

A selection of genomic isolation kits are offered that purify high quality genomic DNA  from a wide variety of sources and for a wide array of applications.

 

Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications. These kits are fully scalable for large genomic DNA isolations. The procedure is slightly more involved to ensure ultra pure DNA.

 

Based on our popular OmniPrep™ system, the OmniPrep™ for Yeast kit isolates high purity, high quality genomic DNA (approximately 100kbp) from yeast. The kit yields three to six micrograms per milliliter yeast culture. If used according to protocol,

 

OmniPrep™ for Yeast purifies DNA from 300 milliliters yeast culture.

 

The OmniPrep™ for Yeast kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. The kit is supplied with a proprietary yeast suspension buffer and our  LongLife™ Zymolyase®  for the rapid resuspension and digestion of the yeast cell walls for DNA release. Pure genomic DNA is isolated in 20 to 40 minutes, depending on the tissue type used.

 

Features

  • High purity DNA: A260/A280 between 1.8 & 2.0
  • Quick and simple two tube method
  • No toxic chemicals; no phenol; no hazardous waste
  • Supplied with yeast suspension buffer & LongLife™ Zymolyase® for efficient DNA release

 

Applications

  • Extraction of pure genomic DNA from yeast tissue
  • Extraction of high quality, 100kb genomic DNA

 

 

 

Additional information

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