PBST [10X]

£77.00£228.00 ex. VAT
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R044-XX
SKU: R044-XX Category:
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Description

A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4.

 

PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.

 

By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

 

Features

  • 1X Concentration is 8mM Na2HPO4, 150mM NaCl, 2mM KH2PO4, 3mM KCl, 0.05% Tween® 20, pH 7.4

 

Applications

  • Wash buffer for Western Blotting and ELISA

Additional information

Size

1 Gallon, 1L

Description

PBST [10X]; Phosphate buffered saline with Tween-20

SKU SizeDescription Price
BER044 1L PBST [10X]; Phosphate buffered saline with Tween-20 £77.00
BER045 1 Gallon PBST [10X]; Phosphate buffered saline with Tween-20 £228.00