picoLUCENT™ PLUS-HRP

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SKU: 786-002-XX Category:
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Description

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

 

picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. 

 

picoLUCENT™ PLUS-HRP reagents are available in five sizes suitable for 5, 25, 50, 125 and 250 blots as each 4ml of working solution is suitable for 1 mini blot (8 x 7.5cm)

 

In addition, picoLUCENT™ PLUS-HRP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP-BLOCKER™) and wash buffer (femto-TBST™).  The picoLUCENT™ PLUS-HRP kits allow detection of low picogram levels (10-12) of antigens with low noise (signal/background) ratio.  The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane.  

 

For a complete chemiluminescence immunodetection system, we offer optional horesradish perxodase (HRP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat. 

 

Features

  • Low picogram detection (10-12)
  • Low Background : High signal ratio
  • 1 year shelf life
  • Reagent for 25, 50 and 125 mini blots
  • Nitrocellulose or PVDF compatible
  • Kit formats supplied with optional HRP or AP conjugated secondary antibodies.

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