Protein A (Recombinant)

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SKU: BE786-1266 Category:
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Description

Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. Protein A contains four high-affinity binding sites for interacting with the Fc region of immunoglobulin Gs of various species including mouse, rabbit and human. Protein A is used for making probes for detection of antibodies and for making affinity media for purification of antibodies.

Protein A (Recombinant) is recombinant protein A expressed in E. coli. It functions similarly as the native Protein A.

Protein A (Recombinant) can be conjugated with reporter molecules, fluorescent dyes, enzyme markers, biotin or colloidal gold for detection of antibodies by immunochemical assays including ELISA, western blotting or Immunohistochemistry.

Protein A (Recombinant) can be immobilized on a solid support such as agarose to purify monoclonal or polyclonal antibodies. 

 

Features:

  • Source: E. coli.
  • Molecular weight: ~47kDa; exact sequence as the native Protein A.
  • Binds 3 IgG Isotypes.
  • Purity: ≥ 95%, HPLC, Single major band.
  • Form: Lyophilized powder.

 

Application(s):

  • Protein A (Recombinant) conjugated to reporter molecules or fluourescent dyes or enzymemarkers can be used in detection of antibody by immunochemicals assays.
  • Protein A (Recombinant)  immobilized on a solid support such as agarose is used to purify monoclonal or polyclonal antibodies. 

Image By E A S (Own work)

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