Proteinase K, 200mg

Key Features

Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable

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SKU: BI-405002 Categories: ,
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Description

Application:
Digestion of proteins
 
Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).

Activity:  > 30 units/mg protein (hemoglobin, pH 7.5, 37°C)

Unit definition:
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.

Features:
Proteinase is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

 

Usage:
In presence of 0.5 – 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

 

Quality of Proteinase K:
– purified by chromatography and lyophilised
– RNases: not detectable
– DNases: not detectable
– Exonucleases: not detectable

Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature