Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure.
G-Biosciences’ proteomic grade detergent solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered as sterile, 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.
The Proteomic Grade Detergent Variety Pack contains a selection of non-ionic proteomic grade detergent solutions, Zwitterionic and non-detergent sulfobetaines for trial and optimization. The pack contains one 10ml vial of 10% aqueous solutions of :
And 1gm of:
- Low peroxide contamination
- Low carbonyl contamination
- Low conductivity
- Sterile detergent solution
- Reduced metal ions
- 10% aqueous solutions
- Sealed under inert gas to prevent oxidation
- Proteomic grade detergent solutions for protein solubilization