Recom ProteaseArrest™

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SKU: 786-376-XX Category:
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Description

A broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases including aminopeptidases and aspartic proteases.

 

Recom ProteaseArrest™ cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca²+, Mg²+ or Mn²+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins.

 

Recom ProteaseArrest™ cocktail is compatible with immobilized metal affinity chromatography.

 

ProteaseArrest Family

Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition.

 

The ProteaseArrest™ family of inhibitor cocktails also includes species specific inhibitor cocktails.  These cocktails are enhanced with inhibitors specific for the species.  Species specific cocktails are available for Bacteria, Plant, Yeast, Fungus and Mammalian samples.

 

Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis.

 

 

Features

  • Protease inhibitor cocktail for greater protection of bacterial expressed recombinant proteins
  • 100X concentrated, ready-to-use solution.

 

 

Applications

  • Inhibition of protease activity in protein preparations of bacterial lysates.
  • Protection of proteins from proteolysis during purification of recombinant proteins.

 

 

Additional information

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