Preventing RNA degradation in the following processes:
– First strand cDNA synthesis
– in vitro transcription and translation
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA, 10 mM DTT and stabilizers.
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.