SG-Lysine-C™

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SKU: BE786-14 Category:
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Description

SG-Lysine-C endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C are chemically modified making the enzyme resistant to autolysis and stabilizing its enzymatic activity.

 

SG-Lysine-C is supplied lyophilized in 20μg vials. The enzyme is typically reconstituted to a concentration of 0.25μg/ml. For fragmentation, the enzyme is added to the sample protein in a ratio of 1:100 to 1:20 (enzyme to protein, by weight) in a standard digestion buffer.

 

Features

  • Modified Lysine-C for sequence analysis.

  • Resistant to autolysis and degradation.

  • High Specificity, free from other known endopeptidases.

  • Consistency of activity levels from lot to lot for reproducible digestions.

  • Recommended SG-Lysine-C™ to protein ratio, 1:100 to 1:20.

 

Applications

  • Digestion of proteins for sequence analysis.

  • Suitable for sequencing applications.

Additional information

Size