SOLIScript 1-step Multiplex Probe Kit (Purple)

Key Features

Description:

SOLIScript 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don’t lose in sensitivity compared to singleplex or duplex assays.

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SKU: 08-61-00250 Categories: ,
Special Requirements?

Description

Applications:

– Detection and quantification of RNA targets with qPCR

– DNA/LNA hydrolysis probe based assays

– Suitable for singleplex and multiplex reactions

 

Reagents Provided:

– 5x One-step Multiplex Probe Mix (Purple)

  HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2), Purple dye

  If Cy5 dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available.

– 40x One-step SOLIScript Mix

  SOLIScript Reverse Transcriptase, RiboGrip RNase Inhibitor

– Water, nuclease free