Description
Applications:
– Detection and quantification of RNA targets with qPCR
– DNA/LNA hydrolysis probe based assays
– Suitable for singleplex and multiplex reactions
Reagents Provided:
– 5x One-step Multiplex Probe Mix (Purple)
HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2), Purple dye
If Cy5 dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available.
– 40x One-step SOLIScript Mix
SOLIScript Reverse Transcriptase, RiboGrip RNase Inhibitor
– Water, nuclease free
