SOLIScript 1-step Multiplex Probe Kit (ROX)

Key Features

Description:

SOLIScript 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don’t lose in sensitivity compared to singleplex or duplex assays.

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SKU: 08-59-00250 Categories: ,
Special Requirements?

Description

Applications:

– Detection and quantification of RNA targets with qPCR

– DNA/LNA hydrolysis probe based assays

– Suitable for singleplex and multiplex reactions

 

Reagents Provided:

– 5x One-step Multiplex Probe Mix (ROX)

  HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2), ROX dye

  If ROX dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.

– 40x One-step SOLIScript Mix

  SOLIScript Reverse Transcriptase, RiboGrip RNase Inhibitor

– Water, nuclease free

 

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.