– Detection and quantification of RNA targets with qPCR
– DNA/LNA hydrolysis probe based assays
– Suitable for singleplex and multiplex reactions
– 5x One-step Multiplex Probe Mix (ROX)
HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2), ROX dye
If ROX dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
– 40x One-step SOLIScript Mix
SOLIScript Reverse Transcriptase, RiboGrip RNase Inhibitor
– Water, nuclease free
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.