– First strand cDNA synthesis
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
– SOLIScript Enzyme Mix
SOLIScript RT and RiboGrip RNase Inhibitor
– 10x RT Reaction Premix without primers
RT Reaction Buffer with DTT and dNTPs
– Water, nuclease free
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.